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Mechanistic Deep Dive
Bilirubin Pathway Details
- Heme oxygenase: heme â biliverdin
- Biliverdin reductase: biliverdin â unconjugated bilirubin
- Albumin transport
- OATP uptake by hepatocyte
- UGT1A1: conjugation with glucuronic acid
- MRP2: canalicular excretion
- Intestinal bacteria: deconjugation â urobilinogen
- 80% excreted as stercobilin
- 20% reabsorbed (enterohepatic)
- Some excreted as urobilin
Cholestasis Mechanisms
- Impaired bile formation (intracellular)
- Impaired transport (canalicular)
- Bile duct destruction (PBC, PSC)
- Extrahepatic obstruction
Recent Trials & Updates
Non-Invasive Fibrosis Assessment
- Increasingly used vs liver biopsy
- ELF + transient elastography combination
- Multi-step algorithms (FIB-4 â FibroScan â biopsy)
MR Elastography
- Most accurate non-invasive
- Costly + limited availability
Vibration-Controlled Transient Elastography
- Point-of-care
- Increasingly available
High-Yield Specialist Points
Macroenzymes in LFTs
- â ALP without GGT increase: bone, placenta
- Confirm liver origin with GGT or isoenzyme
Hereditary Hyperbilirubinemia in IBD
- Gilbert common
- Mistaken for hepatic disease
Pregnancy Effects on LFTs
- ALP rises 2-4x (placenta)
- ALT/AST slight rise
- Albumin falls (dilutional)
Acetaminophen + Alcohol
- Increased toxicity at lower doses
- CYP2E1 induction
- Glutathione depletion
Wilson Disease Workflow
- Young + neurologic / psychiatric / liver disease
- Low ceruloplasmin (< 20)
- Slit-lamp for Kayser-Fleischer rings
- 24-h urinary copper > 100
- Liver biopsy with copper > 250 ÎŒg/g dry weight
- Genetic testing (ATP7B)
Hemochromatosis Workflow
- Iron overload pattern
- Ferritin > 1000, TSAT > 45%
- HFE genotyping (C282Y homozygous most common)
- Liver biopsy for staging (less now with MRI)
- Phlebotomy
Alpha-1 Antitrypsin Workup
- Serum level
- Phenotype (PiZZ severe)
- COPD + liver disease
- Augmentation therapy + liver transplant
Bile Acid Diuretic Test
- For mild persistent cholestasis
- Workup before biopsy
Hepatic Granulomas
- Sarcoidosis, TB, infections, drugs, PBC
- Biopsy + workup
Hepatic Adenoma
- OCP-related (estrogen)
- Bleeding + malignant transformation risk
- Imaging + biopsy + resection if large
Focal Nodular Hyperplasia (FNH)
- Benign
- Central scar on imaging
- No malignant risk
Liver Cysts
- Simple cysts common
- Hydatid (echinococcus)
- Polycystic (ADPKD)
Pearls
- LFT patterns: hepatocellular vs cholestatic vs mixed
- AST/ALT ratio: alcoholic > 2; cirrhosis/muscle > 1
- Bilirubin metabolism: heme â bilirubin â UGT â MRP2 â bile
- Gilbert syndrome: common benign
- Workup: pattern + history + imaging + serology + biopsy
- Non-invasive fibrosis: FIB-4, NFS, ELF, FibroScan, MR elastography
- Acute hepatitis (ALT > 1000): drug, viral, autoimmune, ischemic, Wilson