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Mechanistic Deep Dive
TB Granuloma
- Macrophages, T cells (CD4 Th1 dominant)
- Multinucleated giant cells (Langhans)
- Caseating necrosis
- TNF-α critical
- IFN-γ critical
TB Drug Mechanisms
- Isoniazid: inhibits mycolic acid synthesis (InhA + KatG)
- Rifampin: RNA polymerase (RpoB)
- Pyrazinamide: pyrazinamidase + low pH active in macrophages
- Ethambutol: arabinogalactan (cell wall)
- Streptomycin: 30S ribosome
- Bedaquiline: ATP synthase
- Pretomanid: nitroimidazo-oxazine (DNA + RNA synthesis)
- Linezolid: 50S ribosome (also for MRSA)
Drug Resistance Mechanisms
- INH: KatG mutation (50%); InhA promoter (~ 30%); other
- RIF: RpoB mutation
- Fluoroquinolone: GyrA mutation
- PZA: pncA mutation
- Genotypic testing for rapid detection
Recent Trials & Updates
Nix-TB (2020) â BPaL for MDR/XDR-TB
- N = 109 MDR/XDR-TB + treatment-failure
- BPaL Ã 6 months
- 89% success
- Revolution in MDR-TB treatment
ZeNix (2022)
- Optimized linezolid dose (less toxicity)
- Same efficacy
- Practice-changing
TB-PRACTECAL (2022)
- BPaLM Ã 6 months vs longer standard for MDR-TB
- Superior efficacy + safety
- WHO 2022 endorsement
3HP for LTBI
- Comparable efficacy to 9H
- Shorter, better adherence
- DOT typically
- Preferred over 9H now
iAdhere (2024)
- Adherence monitoring with apps
TB Vaccine Development
- M72/AS01E (Gates Foundation): phase 3
- VPM1002, MTBVAC: phase 2-3
- BCG re-vaccination studies
- Hope for adult vaccine
COVID-19 + TB
- Co-infection â mortality
- TB diagnosis delayed during pandemic
- Resurgence concerns
High-Yield Specialist Points
Diagnosis Algorithm
- Symptoms compatible + risk factors
- CXR or CT
- Sputum AFB smear + culture + NAAT (Xpert MTB/RIF)
- Drug susceptibility testing (DST)
- HIV testing
- Treatment initiation
- Public health reporting
Sputum Conversion Monitoring
- Monthly smear + culture
- Conversion within 2 months expected
- Failure â drug resistance, adherence, malabsorption
- Extended treatment if persistent
TB + Diabetes
- 3-4x higher TB risk
- Both diseases reinforce each other
- Poorer outcomes
- Bidirectional screening
- Aggressive DM control during TB
TB + TNF-α Inhibitors
- 5-10x reactivation risk
- Screen IGRA/TST before starting
- Treat LTBI if positive
- Some guidelines: continue biologic during LTBI treatment
TB Pericarditis
- Effusive â effusive-constrictive â constrictive
- ADA elevated in pericardial fluid
- IMPI trial 2014: adjunctive prednisolone reduced hospitalization + constrictive pericarditis
- Standard 6-month regimen
TB in End-Stage Renal Disease
- Higher risk
- Drug adjustments: rifampin OK, INH OK, EMB adjust (mostly renal excreted), PZA adjust
- Watch ototoxicity (streptomycin)
Rifabutin vs Rifampin
- Less CYP induction
- Useful in HIV + protease inhibitors
- Same TB efficacy
Drug Interactions
- Rifampin induces CYP3A4 â reduces:
- Contraceptives, warfarin, HIV protease inhibitors
- Many other drugs
- Switch to rifabutin or use alternative
Hepatotoxicity Management
- Monitor LFTs monthly
- Hold if ALT > 3x ULN + symptoms OR > 5x ULN asymptomatic
- Re-introduce single drugs to identify culprit
- Substitute non-hepatotoxic drugs (ethambutol + streptomycin + fluoroquinolone)
- Liver consult
Pyridoxine (B6) with INH
- 25-50 mg daily
- Prevents peripheral neuropathy
- Especially in: pregnancy, alcoholism, malnourished, DM, HIV, renal disease, breastfeeding
IRIS Management
- Continue ART + TB treatment
- Steroids for severe (Meintjes 2010)
- NSAIDs for mild
- Donât stop therapies
Surgery in TB
- For drug-resistant (MDR/XDR) refractory disease
- Selected
- Combined with multidrug therapy
TB-Lung Cancer Association
- TB scar carcinoma (rare association)
- Surveillance in TB survivors
Pearls
- AFB smear + culture + Xpert MTB/RIF for active TB diagnosis
- IGRA preferred for LTBI in BCG-vaccinated (Taiwan)
- RIPE Ã 2 mo â RI Ã 4 mo standard
- MDR-TB: BPaL/BPaLM Ã 6 months (Nix-TB, ZeNix, TB-PRACTECAL)
- 3HP (rifapentine + INH weekly à 12 wk) preferred for LTBI
- HIV + TB: ART within 2 weeks if low CD4; watch IRIS
- TB meningitis: 12 mo + steroids
- Reportable disease + airborne isolation + DOT
- Ethambutol: optic neuritis (check vision)
- Pyridoxine with INH