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Mechanistic Deep Dive
Podocyte Biology
- Foot processes + slit diaphragm (nephrin, podocin)
- Maintain filtration barrier
- Injury â foot process effacement â proteinuria
- MCD, FSGS, MN all involve podocyte damage (different mechanisms)
Anti-PLA2R Discovery (2009)
- Phospholipase A2 receptor
- Target antigen on podocytes
- 70-80% of primary MN
- THSD7A + NELL-1 minor antibodies
Complement in GN
- C3G: alternative pathway dysregulation
- Eculizumab: terminal complement (C5)
- Iptacopan: factor B (alternative pathway)
ANCA Pathophysiology
- ANCA binds neutrophil antigens
- TNF-primed neutrophils
- Neutrophil degranulation â endothelial damage
- Crescent formation
Recent Trials & Updates
MENTOR (2019) â Rituximab MN
- Rituximab vs cyclosporine
- Better remission + safer
- First-line for primary MN
TESTING (2017, 2022) â Methylprednisolone IgA
- Oral methylprednisolone in high-risk IgA
- Reduced eGFR decline
- Safety concerns
DUPLEX (2023) â Sparsentan FSGS
- â Proteinuria vs irbesartan
- FDA approved 2023 (FSGS)
PROTECT (2023) â Sparsentan IgA
- â Proteinuria vs irbesartan
- FDA approved for IgA 2023
NefigaRD (2021) â Nefecon IgA
- Targeted-release budesonide
- â Proteinuria + eGFR preservation
- FDA approved 2021
APPLAUSE-IgAN (Ongoing) â Iptacopan
- Factor B inhibitor
- Promising results
BLISS-LN (2020) â Belimumab Lupus
- BAFF inhibitor
- Added to standard
- â Renal response
AURORA (2020) â Voclosporin Lupus
- New CNI
- More potent
- â Proteinuria added to MMF
PEXIVAS (2020) â Plasmapheresis + Steroid Dose ANCA
- Plasmapheresis benefit limited (subgroups)
- Reduced steroid dose acceptable
ADVOCATE (2021) â Avacopan ANCA
- C5a receptor antagonist
- Steroid-sparing
- FDA approval 2021
Inaxaplin (AMPLITUDE Ongoing) â APOL1
- Targeted for APOL1 nephropathy
- Phase 3 trial
ANDROMEDA (2021) â Dara-VCD AL Amyloid
- Daratumumab + bortezomib + cyclophosphamide + dexamethasone
- Front-line for AL amyloid
High-Yield Specialist Points
MCD Adult Specifics
- Higher relapse + steroid resistance
- More cytotoxic IS needed
- Adult variants: think lithium, NSAIDs, Hodgkin
- Rituximab effective for relapsers
FSGS Recurrence Post-Transplant
- Primary FSGS: 20-30% recurrence
- Within hours-days
- Plasmapheresis pre/post-transplant
- ICZ apheresis, rituximab for severe
MN Anti-PLA2R Monitoring
- Levels correlate with disease activity
- Decline predicts treatment response
- Persistent positivity suggests need for further IS
IgA Nephropathy MEST-C Scoring
- Oxford classification
- Prognostic value
- Guides treatment intensity
Lupus Nephritis Class V Pure
- Mostly nephrotic
- Treatment: supportive (RAAS) ± IS if severe
ANCA-Associated Renal-Limited Vasculitis
- Some cases isolated renal
- Same IS approach
Hereditary GN Considerations
- Alport syndrome: type IV collagen
- Thin GBM disease: COL4A3/4 heterozygous
- Genetic testing
- Family screening
- Hearing loss + eye findings in Alport
Drug-Induced GN
- NSAIDs: MCD, AIN
- ICI: ATN, AIN, MCD, MN, lupus nephritis-like
- Penicillamine: MN, ANCA
- Hydralazine: lupus-like, ANCA
- Pamidronate: collapsing FSGS
Cancer-Associated GN
- MN: solid tumors (colon, lung, breast, prostate)
- MCD: Hodgkin
- AL amyloid: myeloma
- Cryoglobulinemia: lymphoma, hep C
- Membranoproliferative: monoclonal gammopathy
Pregnancy + GN
- Lupus nephritis particularly important
- IS adjustment (avoid MMF, cyclophosphamide; use AZA)
- Plasma exchange OK for severe
- Multidisciplinary
Renal Biopsy in Specific Conditions
- Hep C + NS: MPGN, MN
- HIV + NS: FSGS (collapsing), HIVAN
- Older adult NS: rule out MN; consider amyloid + monoclonal
- AKI on top of CKD: consider drug-induced AIN, RPGN
Avacopan Use
- ANCA-associated vasculitis
- Steroid-sparing
- Long-term safety being assessed
- Approved adjunct
Sparsentan Practical Use
- Dual ETA + AT1R antagonist
- Replace ACE/ARB
- â Proteinuria
- Monitor: BP, K, Cr
- BP may be lower than expected
Iptacopan Mechanism
- Factor B inhibitor (alternative complement)
- Oral
- For C3G + IgA emerging
- Phase 3 trials
Pearls
- MCD: kids, steroids; foot process effacement
- FSGS: adults US; APOL1; collapsing variant worst
- MN: anti-PLA2R 70-80%; rituximab MENTOR; renal vein thrombosis
- IgA: globally most; synpharyngitic; Nefecon FDA 2021
- Lupus: ISN/RPS I-VI; MMF + steroids + voclosporin/belimumab
- ANCA: pauci-immune; rituximab + steroids + avacopan FDA 2021
- Anti-GBM: plasmapheresis + cyclophosphamide + steroids
- MPGN/C3G: iptacopan emerging
- DKD: 4 pillars
- AL amyloid: daratumumab + CyBorD