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1ïžâ£ VL Returning Traveler / Immigrant
- Prolonged fever + massive splenomegaly + pancytopenia in immigrant from endemic â consider VL
- rK39 rapid test screening
- Bone marrow aspirate definitive
- Hematology + ID consultation
- Hospitalization + treatment
2ïžâ£ Bone Marrow vs Splenic Aspirate
- Bone marrow: standard, sensitivity 60-85%, safe
- Splenic aspirate: highest sensitivity (95%) but bleeding risk
- Reserved for treatment failures or refractory diagnosis
- Imaging-guided splenic aspirate to minimize bleeding
3ïžâ£ Liposomal AmB Single Dose Strategy
- 10 mg/kg single IV dose for Indian VL (WHO endorsement)
- Used in mass campaigns + India elimination
- Safer than antimony (less cardiac toxicity)
- Cost-effectiveness analyses positive
- Adopted in endemic countries
4ïžâ£ MCL Workup + Treatment
- Always systemic treatment (not local)
- Pentavalent antimony à 28+ days or liposomal AmB
- Surgical reconstruction after parasite cure
- Ophthalmology / ENT involvement for complications
- Long follow-up + counseling
5ïžâ£ PKDL Implications
- Reservoir â continued transmission
- L. donovani anthroponotic regions
- Treatment of PKDL â outbreak control
- WHO recommendation to identify + treat all PKDL
6ïžâ£ HIV + VL Co-Infection Algorithm
- More severe initial presentation
- Atypical features (less fever, atypical lab)
- Relapsing course common
- Treatment: liposomal AmB 40 mg/kg total (longer)
- Maintenance / secondary prophylaxis: monthly liposomal AmB until CD4 > 350 sustained
- ART critical
7ïžâ£ India Elimination Program
- WHO 2030 target
- Mass screening + treatment
- Liposomal AmB mass deployment
- Vector control + bed nets
- PKDL identification + treatment
- 90%+ reduction in cases over past decade
8ïžâ£ å¥ä¿ / Taiwan
- çœèŠ endemic; imported cases
- éå ± mandatory
- å¥ä¿ antimonials, miltefosine, liposomal AmB for confirmed cases
- ID consult + reference labs
9ïžâ£ Sandfly + Climate Change
- Sandfly range expanding northward (Mediterranean basin spreading north Europe)
- Climate change impact on transmission
- Surveillance ongoing
- Increasing autochthonous CL cases in N Italy + S France
10. Future + Vaccines
- LEISH-F2 (recombinant) â Phase 2/3
- LEISH-F3 candidates
- Whole-killed + DNA + mRNA candidates
- ChAd vector vaccines
- Combined CL + VL vaccines researched